Using superfolder green fluorescent protein for periplasmic protein localization studies.
نویسندگان
چکیده
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.
منابع مشابه
Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila
BACKGROUND A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila. RESULTS In vivo fluorescence spectroscopic analyses of clones ca...
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عنوان ژورنال:
- Journal of bacteriology
دوره 193 18 شماره
صفحات -
تاریخ انتشار 2011